Bicinchoninic Acid Protein Assay:Article Title: 1-deoxysphingolipids dysregulate membrane properties and cargo trafficking in the early secretory pathway.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays PierceTM BCA protein assay Thermo Fisher Scientific Cat# 23225 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 Seahorse Cell Mito Stress Test Agilent Cat# 103015 Experimental models: Cell lines hTERT-RPE-1 cells ATCC ID# CRL-4000 RPE-1 SPT WT This paper N/A RPE-1 SPT C133W This paper N/A Recombinant DNA mCherry-Sec23A Weigel et al. 45 Addgene #166894 mCherry-Sec61β J. Lippincott-Schwartz N/A mApple-Sec61β J. Lippincott-Schwartz N/A Galactosyltransferase-mApple (GalTmApple) J. Lippincott-Schwartz N/A Sialyl Transferase-mApple (SiT-mApple) J. Lippincott-Schwartz N/A pGEX-4-T1 Genscript Cat# V010918 pGEX-4-T1 CERT START This study N/A Str-KDEL_TfR-SBP-mCherry (TfRmCherry) Weigel et al. 45 N/A Str-KDEL_SBP-mApple-Ecadherin J. Lippincott-Schwartz N/A Str-KDEL_TNF-SBP-mCherry (TNFmCherry) Boncompain et al. 59 Addgene #65279 Str-KDEL_SBP-mCherry-GPI (mCherry-GPI) Boncompain et al. 59 Addgene #65295 Str-KDEL_SBP-mCherry-Gp135 (mCherryGp135-RUSH) Weigel et al. 45 N/A Software and algorithms ImageJ/Fiji version 2.0.0-rc-69/1.53o National Institutes of Health https://imagej.net/software/fiji/downloads GraphPad Prism version 10.1.1 GraphPad Software https://www.graphpad.com/updates/ prism-10-1-1-release-notes ZEN Black version 2.3 ZEISS https://www.micro-shop.zeiss.com/en/us/ softwarefinder/software-categories/zenblack/zen-black-system/ Python 3.15 Python Software Foundation https://pypi.org/ LaurdanSegmentation This paper Zenodo: https://doi.org/10.5281/zenodo. .. 17874908 Other 35 mm glass bottom dishes No 1.5 Cover Slip MatTek Cat# P35G-1.5-14-C Glutathione SepharoseTM 4B GSTtagged resin Cytiva Cat# 17075605 Amicon Ultra 15 MWCO 10 kDa concentrator Millipore Cat# UFC9010 Slide-A-Lyzer MWCO 3.5 kDa Thermo Fisher Scientific Cat# A52966 mini-PROTEAN TGX gels Bio-Rad Cat# 4568026 Cell Chemical Biology 33, 45–58.e1–e8, January 15, 2026 e3 received by the supplier, but regular cell morphology, Golgi apparatus sturcutre, and monolayer formation capacity of RPE-1 was regularly checked.
Single-particle Tracking:Article Title: 1-deoxysphingolipids dysregulate membrane properties and cargo trafficking in the early secretory pathway.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays PierceTM BCA protein assay Thermo Fisher Scientific Cat# 23225 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 Seahorse Cell Mito Stress Test Agilent Cat# 103015 Experimental models: Cell lines hTERT-RPE-1 cells ATCC ID# CRL-4000 RPE-1 SPT WT This paper N/A RPE-1 SPT C133W This paper N/A Recombinant DNA mCherry-Sec23A Weigel et al. 45 Addgene #166894 mCherry-Sec61β J. Lippincott-Schwartz N/A mApple-Sec61β J. Lippincott-Schwartz N/A Galactosyltransferase-mApple (GalTmApple) J. Lippincott-Schwartz N/A Sialyl Transferase-mApple (SiT-mApple) J. Lippincott-Schwartz N/A pGEX-4-T1 Genscript Cat# V010918 pGEX-4-T1 CERT START This study N/A Str-KDEL_TfR-SBP-mCherry (TfRmCherry) Weigel et al. 45 N/A Str-KDEL_SBP-mApple-Ecadherin J. Lippincott-Schwartz N/A Str-KDEL_TNF-SBP-mCherry (TNFmCherry) Boncompain et al. 59 Addgene #65279 Str-KDEL_SBP-mCherry-GPI (mCherry-GPI) Boncompain et al. 59 Addgene #65295 Str-KDEL_SBP-mCherry-Gp135 (mCherryGp135-RUSH) Weigel et al. 45 N/A Software and algorithms ImageJ/Fiji version 2.0.0-rc-69/1.53o National Institutes of Health https://imagej.net/software/fiji/downloads GraphPad Prism version 10.1.1 GraphPad Software https://www.graphpad.com/updates/ prism-10-1-1-release-notes ZEN Black version 2.3 ZEISS https://www.micro-shop.zeiss.com/en/us/ softwarefinder/software-categories/zenblack/zen-black-system/ Python 3.15 Python Software Foundation https://pypi.org/ LaurdanSegmentation This paper Zenodo: https://doi.org/10.5281/zenodo. .. 17874908 Other 35 mm glass bottom dishes No 1.5 Cover Slip MatTek Cat# P35G-1.5-14-C Glutathione SepharoseTM 4B GSTtagged resin Cytiva Cat# 17075605 Amicon Ultra 15 MWCO 10 kDa concentrator Millipore Cat# UFC9010 Slide-A-Lyzer MWCO 3.5 kDa Thermo Fisher Scientific Cat# A52966 mini-PROTEAN TGX gels Bio-Rad Cat# 4568026 Cell Chemical Biology 33, 45–58.e1–e8, January 15, 2026 e3 received by the supplier, but regular cell morphology, Golgi apparatus sturcutre, and monolayer formation capacity of RPE-1 was regularly checked.
Recombinant:Article Title: 1-deoxysphingolipids dysregulate membrane properties and cargo trafficking in the early secretory pathway.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays PierceTM BCA protein assay Thermo Fisher Scientific Cat# 23225 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 Seahorse Cell Mito Stress Test Agilent Cat# 103015 Experimental models: Cell lines hTERT-RPE-1 cells ATCC ID# CRL-4000 RPE-1 SPT WT This paper N/A RPE-1 SPT C133W This paper N/A Recombinant DNA mCherry-Sec23A Weigel et al. 45 Addgene #166894 mCherry-Sec61β J. Lippincott-Schwartz N/A mApple-Sec61β J. Lippincott-Schwartz N/A Galactosyltransferase-mApple (GalTmApple) J. Lippincott-Schwartz N/A Sialyl Transferase-mApple (SiT-mApple) J. Lippincott-Schwartz N/A pGEX-4-T1 Genscript Cat# V010918 pGEX-4-T1 CERT START This study N/A Str-KDEL_TfR-SBP-mCherry (TfRmCherry) Weigel et al. 45 N/A Str-KDEL_SBP-mApple-Ecadherin J. Lippincott-Schwartz N/A Str-KDEL_TNF-SBP-mCherry (TNFmCherry) Boncompain et al. 59 Addgene #65279 Str-KDEL_SBP-mCherry-GPI (mCherry-GPI) Boncompain et al. 59 Addgene #65295 Str-KDEL_SBP-mCherry-Gp135 (mCherryGp135-RUSH) Weigel et al. 45 N/A Software and algorithms ImageJ/Fiji version 2.0.0-rc-69/1.53o National Institutes of Health https://imagej.net/software/fiji/downloads GraphPad Prism version 10.1.1 GraphPad Software https://www.graphpad.com/updates/ prism-10-1-1-release-notes ZEN Black version 2.3 ZEISS https://www.micro-shop.zeiss.com/en/us/ softwarefinder/software-categories/zenblack/zen-black-system/ Python 3.15 Python Software Foundation https://pypi.org/ LaurdanSegmentation This paper Zenodo: https://doi.org/10.5281/zenodo. .. 17874908 Other 35 mm glass bottom dishes No 1.5 Cover Slip MatTek Cat# P35G-1.5-14-C Glutathione SepharoseTM 4B GSTtagged resin Cytiva Cat# 17075605 Amicon Ultra 15 MWCO 10 kDa concentrator Millipore Cat# UFC9010 Slide-A-Lyzer MWCO 3.5 kDa Thermo Fisher Scientific Cat# A52966 mini-PROTEAN TGX gels Bio-Rad Cat# 4568026 Cell Chemical Biology 33, 45–58.e1–e8, January 15, 2026 e3 received by the supplier, but regular cell morphology, Golgi apparatus sturcutre, and monolayer formation capacity of RPE-1 was regularly checked.
Software:Article Title: 1-deoxysphingolipids dysregulate membrane properties and cargo trafficking in the early secretory pathway.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays PierceTM BCA protein assay Thermo Fisher Scientific Cat# 23225 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 Seahorse Cell Mito Stress Test Agilent Cat# 103015 Experimental models: Cell lines hTERT-RPE-1 cells ATCC ID# CRL-4000 RPE-1 SPT WT This paper N/A RPE-1 SPT C133W This paper N/A Recombinant DNA mCherry-Sec23A Weigel et al. 45 Addgene #166894 mCherry-Sec61β J. Lippincott-Schwartz N/A mApple-Sec61β J. Lippincott-Schwartz N/A Galactosyltransferase-mApple (GalTmApple) J. Lippincott-Schwartz N/A Sialyl Transferase-mApple (SiT-mApple) J. Lippincott-Schwartz N/A pGEX-4-T1 Genscript Cat# V010918 pGEX-4-T1 CERT START This study N/A Str-KDEL_TfR-SBP-mCherry (TfRmCherry) Weigel et al. 45 N/A Str-KDEL_SBP-mApple-Ecadherin J. Lippincott-Schwartz N/A Str-KDEL_TNF-SBP-mCherry (TNFmCherry) Boncompain et al. 59 Addgene #65279 Str-KDEL_SBP-mCherry-GPI (mCherry-GPI) Boncompain et al. 59 Addgene #65295 Str-KDEL_SBP-mCherry-Gp135 (mCherryGp135-RUSH) Weigel et al. 45 N/A Software and algorithms ImageJ/Fiji version 2.0.0-rc-69/1.53o National Institutes of Health https://imagej.net/software/fiji/downloads GraphPad Prism version 10.1.1 GraphPad Software https://www.graphpad.com/updates/ prism-10-1-1-release-notes ZEN Black version 2.3 ZEISS https://www.micro-shop.zeiss.com/en/us/ softwarefinder/software-categories/zenblack/zen-black-system/ Python 3.15 Python Software Foundation https://pypi.org/ LaurdanSegmentation This paper Zenodo: https://doi.org/10.5281/zenodo. .. 17874908 Other 35 mm glass bottom dishes No 1.5 Cover Slip MatTek Cat# P35G-1.5-14-C Glutathione SepharoseTM 4B GSTtagged resin Cytiva Cat# 17075605 Amicon Ultra 15 MWCO 10 kDa concentrator Millipore Cat# UFC9010 Slide-A-Lyzer MWCO 3.5 kDa Thermo Fisher Scientific Cat# A52966 mini-PROTEAN TGX gels Bio-Rad Cat# 4568026 Cell Chemical Biology 33, 45–58.e1–e8, January 15, 2026 e3 received by the supplier, but regular cell morphology, Golgi apparatus sturcutre, and monolayer formation capacity of RPE-1 was regularly checked.
|
ATCC is a verified supplier 
ATCC manufactures this product 